Virus:Article Title: A mobile CRISPRi collection enables genetic interaction studies for the essential genes of Escherichia coli
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli MFDpir Ferrières et al.50 N/A E. coli Keio gene deletion collection Baba et al.4 N/A E. coli BW25113 Baba et al.4 N/A Chemicals, peptides, and recombinant proteins Anhydrotetracycline Sigma-Aldrich Cat#37919 Spectinomycin Sigma-Aldrich Cat#S4014 Kanamycin Sigma-Aldrich Cat#60615 LOLCDE-in-1 MedChem Express CatHY-130839 MRL-494 GLPBio Cat#GC60254 Ampicillin Sigma-Aldrich Cat#A0166 Colistin Sigma-Aldrich Cat#C4461 Fosfomycin Sigma-Aldrich Cat#34089 Diaminopimelic acid (DAP) Fisher Scientific Cat#AAB2239103 MOPS-minimal media TekNova Cat#M2101 LB Broth, Miller Fisher Scientific Cat#DF0446 Agar Fisher Scientific Cat#BP1423 Deposited data Plate images and data from Dlpp CRISPRi Cross and pFD152-lolA Keio Cross This work https://doi.org/10.5281/zenodo.10214517 Oligonucleotides Oligonucleotides used in this work This paper See Table S1 Recombinant DNA pFD152 Depardieu and Bikard.44 Addgene Cat#125546 Software and algorithms GraphPad PRISM 9 GraphPad https://www.graphpad.com/ CRISPRbact sgRNA design tool Cui et al.34 https://gitlab.pasteur.fr/dbikard/crisprbact ImageJ ImageJ https://ImageJ.net/ij/ij/ ImageJ colony biomass quantification script French et al.12 https://doi.org/10.5281/zenodo.10214517 Analysis R code for Dlpp CRISPRi cross and CRISPRi Keio Crosses This work https://doi.org/10.5281/zenodo.10214517 Other Epson Perfection V750 high-resolution scanner Epson NA Singer ROTOR+ Singer Instruments https://www.singerinstruments.com/solution/rotor/ Singer RePads (384 Long and 1536 short) Singer Instruments Cat#REP-003; Cat#REP-005 Singer PlusPlates Singer Instruments Cat#PLU-003 Biotek Synergy Neo2 Plate Reader BioTek N/A ..
Article Title: STAT3 couples activated tyrosine kinase signaling to the oncogenic core transcriptional regulatory circuitry of anaplastic large cell lymphoma
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BATF3 R&D systems Cat#AF7437; RRID: AB_11127798 BRD4 (E2A7X) Cell Signaling Cat#13440; RRID: AB_2687578 H3K27ac Abcam Cat#ab4729; RRID: AB_2118291 IKZF1 (D6N9Y) Cell Signaling Cat#14859; RRID: AB_2744523 IRF4 (D6P5H) Cell Signaling Cat#15106; RRID: AB_2798709 MED1 Bethyl Laboratories Cat#A300-793A; RRID: AB_577241 MYC Cell Signaling Cat#5605; RRID: AB_1903938 STAT3 (D3Z2G) Cell Signaling Cat#126403; RRID: AB_2799645 Mouse IgG Santa Cruz Biotechnology Cat#sc-2025; RRID: AB_737182 H3K27me3 Abcam Cat#ab192985; RRID: AB_2650559 CUTANA Rabbit IgG Epicypher Cat#130042; RRID: AB_2923178 p-STAT3 (Y705) Cell Signaling Cat#9145; RRID: AB_2491009 STAT3 (124H6) Cell Signaling Cat#9139; RRID: AB_331757 ACTB Cell Signaling Cat#4970; RRID: AB_2223172 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Life Technologies Cat#32460; RRID: AB_1185567 Goat anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#62-6520; RRID: AB_2533947 Bacterial and virus strains One shot Stbl 3 Chemically Competent E. coli Invitrogen Cat#C737303 Biological samples Patient-derived xenografts (PDX) ALCL sample DFCI CPDM WCTL-81162-Q13 Patient tumor (ALCL) Liang et al.53 Chemicals, peptides, and recombinant proteins Crizotinib Medchem Express CatHY-50878 Ruxolitinib Medchem Express Cat#HY-50856 Alectinib Medchem Express Cat#HY-13011 Thalidomide Medchem Express Cat#HY-14658 Lenalidomide Medchem Express Cat#HY-A0003 Avadomide Medchem Express Cat#HY-100507 Iberidomide Medchem Express Cat#HY-101291 STAT3-IN-3 Medchem Express Cat#HY-128588 Stattic Medchem Express Cat#HY-13818 FuGene HD Promega Cat#E2311 Sodium chloride Sigma Aldrich Cat#S9888 Formaldehyde Sigma Aldrich Cat#252549 RNase Thermo Fisher Scientific Cat#EN0531 Triton X-100 Sigma Aldrich Cat#T8787 DMSO Fisher Scientific Cat#BP231-100 HEPES Life Technologies Cat#15630080 CaCl2 Thermo Fisher Scientific Cat#AAJ63122AE MnCl2 Sigma Aldrich Cat#M1028 Spermidine trihydrochloride Sigma Aldrich Cat#S2501 (Continued on next page) Cell Reports Medicine 5, 101472, March 19, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Digitonin Sigma Aldrich Cat#300410 EDTA Thermo Fisher Scientific Cat#AM9260G EGTA Westnet.
Article Title: A screen for cell envelope stress uncovers an inhibitor of prolipoprotein diacylglyceryl transferase, Lgt, in Escherichia coli .
Article Snippet: .. Bacterial and virus strains Refer to Data S7 This Work N/A Chemicals, peptides, and recombinant proteins Anhydrotetracycline Sigma-Aldrich Cat#37919 Spectinomycin Sigma-Aldrich Cat#S4014 Kanamycin Sigma-Aldrich Cat#60615 LOLCDE-in-1 MedChem Express CatHY-130839 Chloramphenicol Sigma-Aldrich Cat#C0378 CHIR-090 Sigma-Aldrich Cat#SML3092 SPR741 Spero Therapeutics and Northern Antibiotics N/A Diaminopimelic acid (DAP) Fisher Scientific Cat#AAB2239103 MOPS-minimal media TekNova Cat#M2101 .. from Sigma-Aldrich unless otherwise stated Sigma-Aldrich Variable Hexafluoroisopropylsulfamate (HFIPS) Enamine Cas# [637772-38-4] Dess-Martin periodinane AK Scientific Cas# [87413-09-0] 3-quinolinecarboxylic acid AK Scientific Cas# [6480-68-8] 2-picolinic acid AK Scientific Cas# [98-98-6] 4-(methylamino)benzoic acid AK Scientific Cas# [10541-83-0] 3,5-dimethoxybenzoic acid AK Scientific Cas# [1132-21-4] 2-thiophenecarboxaldehyde AK Scientific Cas# [98-03-3] 2-fluoro-4-methoxybenzoic acid AK Scientific Cas# [394-42-3] 3-amino-4-methoxybenzoic acid AK Scientific Cas# [2840-26-8] 4-(trifluoromethyl)benzoic acid AK Scientific Cas# [455-24-3] EDC$HCl AK Scientific Cas# [25952-53-8] DMAP AK Scientific Cas# [1122-58-3] Acetylthiophene Oakwood Chemical Cas# [88-15-3] hexafluoroisopropanol Oakwood Chemical Cas# [920-66-1] 2-Propionylthiophene AmBeed Cas# [13679-75-9] 4-chlorobenzoic acid AmBeed Cas# [74-11-3] 1-methyl-1H-imidazole-5-carboxylic acid AmBeed Cas# [41806-40-0] 2-pivaloylthiophene AmBeed Cas# [20409-48-7] 4-methoxybenzoic acid AmBeed Cas# [100-09-4]
Recombinant:Article Title: A mobile CRISPRi collection enables genetic interaction studies for the essential genes of Escherichia coli
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli MFDpir Ferrières et al.50 N/A E. coli Keio gene deletion collection Baba et al.4 N/A E. coli BW25113 Baba et al.4 N/A Chemicals, peptides, and recombinant proteins Anhydrotetracycline Sigma-Aldrich Cat#37919 Spectinomycin Sigma-Aldrich Cat#S4014 Kanamycin Sigma-Aldrich Cat#60615 LOLCDE-in-1 MedChem Express CatHY-130839 MRL-494 GLPBio Cat#GC60254 Ampicillin Sigma-Aldrich Cat#A0166 Colistin Sigma-Aldrich Cat#C4461 Fosfomycin Sigma-Aldrich Cat#34089 Diaminopimelic acid (DAP) Fisher Scientific Cat#AAB2239103 MOPS-minimal media TekNova Cat#M2101 LB Broth, Miller Fisher Scientific Cat#DF0446 Agar Fisher Scientific Cat#BP1423 Deposited data Plate images and data from Dlpp CRISPRi Cross and pFD152-lolA Keio Cross This work https://doi.org/10.5281/zenodo.10214517 Oligonucleotides Oligonucleotides used in this work This paper See Table S1 Recombinant DNA pFD152 Depardieu and Bikard.44 Addgene Cat#125546 Software and algorithms GraphPad PRISM 9 GraphPad https://www.graphpad.com/ CRISPRbact sgRNA design tool Cui et al.34 https://gitlab.pasteur.fr/dbikard/crisprbact ImageJ ImageJ https://ImageJ.net/ij/ij/ ImageJ colony biomass quantification script French et al.12 https://doi.org/10.5281/zenodo.10214517 Analysis R code for Dlpp CRISPRi cross and CRISPRi Keio Crosses This work https://doi.org/10.5281/zenodo.10214517 Other Epson Perfection V750 high-resolution scanner Epson NA Singer ROTOR+ Singer Instruments https://www.singerinstruments.com/solution/rotor/ Singer RePads (384 Long and 1536 short) Singer Instruments Cat#REP-003; Cat#REP-005 Singer PlusPlates Singer Instruments Cat#PLU-003 Biotek Synergy Neo2 Plate Reader BioTek N/A ..
Article Title: STAT3 couples activated tyrosine kinase signaling to the oncogenic core transcriptional regulatory circuitry of anaplastic large cell lymphoma
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BATF3 R&D systems Cat#AF7437; RRID: AB_11127798 BRD4 (E2A7X) Cell Signaling Cat#13440; RRID: AB_2687578 H3K27ac Abcam Cat#ab4729; RRID: AB_2118291 IKZF1 (D6N9Y) Cell Signaling Cat#14859; RRID: AB_2744523 IRF4 (D6P5H) Cell Signaling Cat#15106; RRID: AB_2798709 MED1 Bethyl Laboratories Cat#A300-793A; RRID: AB_577241 MYC Cell Signaling Cat#5605; RRID: AB_1903938 STAT3 (D3Z2G) Cell Signaling Cat#126403; RRID: AB_2799645 Mouse IgG Santa Cruz Biotechnology Cat#sc-2025; RRID: AB_737182 H3K27me3 Abcam Cat#ab192985; RRID: AB_2650559 CUTANA Rabbit IgG Epicypher Cat#130042; RRID: AB_2923178 p-STAT3 (Y705) Cell Signaling Cat#9145; RRID: AB_2491009 STAT3 (124H6) Cell Signaling Cat#9139; RRID: AB_331757 ACTB Cell Signaling Cat#4970; RRID: AB_2223172 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Life Technologies Cat#32460; RRID: AB_1185567 Goat anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#62-6520; RRID: AB_2533947 Bacterial and virus strains One shot Stbl 3 Chemically Competent E. coli Invitrogen Cat#C737303 Biological samples Patient-derived xenografts (PDX) ALCL sample DFCI CPDM WCTL-81162-Q13 Patient tumor (ALCL) Liang et al.53 Chemicals, peptides, and recombinant proteins Crizotinib Medchem Express CatHY-50878 Ruxolitinib Medchem Express Cat#HY-50856 Alectinib Medchem Express Cat#HY-13011 Thalidomide Medchem Express Cat#HY-14658 Lenalidomide Medchem Express Cat#HY-A0003 Avadomide Medchem Express Cat#HY-100507 Iberidomide Medchem Express Cat#HY-101291 STAT3-IN-3 Medchem Express Cat#HY-128588 Stattic Medchem Express Cat#HY-13818 FuGene HD Promega Cat#E2311 Sodium chloride Sigma Aldrich Cat#S9888 Formaldehyde Sigma Aldrich Cat#252549 RNase Thermo Fisher Scientific Cat#EN0531 Triton X-100 Sigma Aldrich Cat#T8787 DMSO Fisher Scientific Cat#BP231-100 HEPES Life Technologies Cat#15630080 CaCl2 Thermo Fisher Scientific Cat#AAJ63122AE MnCl2 Sigma Aldrich Cat#M1028 Spermidine trihydrochloride Sigma Aldrich Cat#S2501 (Continued on next page) Cell Reports Medicine 5, 101472, March 19, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Digitonin Sigma Aldrich Cat#300410 EDTA Thermo Fisher Scientific Cat#AM9260G EGTA Westnet.
Article Title: STING-OPTN signaling confers cytoprotection through TBK1-dependent mitophagy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CCCP Sigma-Aldrich Cat#C2759 CCCP AmBeed Cat#A806566 Oligomycin Sigma-Aldrich Cat#11342 Antimycin A Sigma-Aldrich Cat#A8674 MG-132 Merck Millipore Cat#474790 Bafilomycin A1 Sigma-Aldrich Cat#B1793 Chloroquine Sigma-Aldrich Cat#C6628 NMS873 Sigma-Aldrich Cat#SML1128-5MG CB5083 MedChem Express CatHY-12861 CsA MedChem Express Cat#HY-B0579 VBIT4 MedChem Express Cat#HY-129122 BAI1 AmBeed Cat#A247314 RU.521 MedChem Express Cat#HY-114180 G150 AmBeed Cat#A1264491 C-170 MedChem Express Cat#HY-138682 C-171 MedChem Express Cat#HY-138683 GSK8612 MedChem Express Cat#HY-111941 Ferrostatin-1 MedChem Express Cat#HY-100579 Necrostatin-1 MedChem Express Cat#HY-15760 Z-VAD-FMK MedChem Express Cat#HY-16658B NAC Sigma-Aldrich Cat#A9165 LysoTrackerTM Deep Red ThermoFisher Cat#L12492 MitoSOX Red MedChem Express Cat#HY-D1055 Critical commercial assays BCA Protein Assay Kit New Cell & MolecularBiotech Cat#WB6501 ECL Chemiluminescent Substrate Reagent Kit Biosharp Cat#BL523B EndoFree Plasmid Midi Kit CWBIO Cat#CW2105S Gel Extraction Kit CWBIO Cat#CW2302M DNA Clean-up Kit CWBIO Cat#CW2301M NanoTransTM Transfection Reagent Plus CYTOCH Cat#CT0005 Lipofectamine 2000 Invitrogen Cat#52887 Lipofectamine 3000 Invitrogen Cat#100022052 Lipofectamine RNAiMAX Invitrogen Cat#56532 ClonExpress® II One Step Cloning Kit Vazyme Biotech Co., Ltd. Cat#C112 Cell Mitochondria Isolation Kit Beyotime Cat#C3601 Experimental models: Cell lines HeLa ATCC Cat#CCL-2; RRID:CVCL_0030 HeLa stably expressing YFP-Parkin (YPH) Dr. Richard J. Youle N/A YPH-cGAS KO This lab N/A YPH-STING KO This lab N/A YPH-TBK1 KO This lab N/A YPH-PINK1 KO Dr. Liming Wang N/A HeLa 3 plus Dr. Han-Ming Shen N/A HeLa 3 plus-cGAS KO This lab N/A HeLa 3 plus-STING KO This lab N/A HeLa 3 plus-TBK1 KO This lab N/A (Continued on next page) 18 Cell Reports 45, 117515, June 23, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER YPH mito-keima Dr. Richard J. Youle N/A HEK293T stably expressing YFP-Parkin Dr. Han-Ming Shen N/A SH-SY5Y ATCC Cat#CRL-2266; RRID:CVCL_0019 Oligonucleotides siRNA targeting cGAS Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.MB21D1.13 siRNA targeting TBK1 Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.TBK1.13 siRNA targeting STING #1: AGCATTACAACAACCTGCT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting STING #2: CGAACTTACAATCAGCATT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting OPTN: CCACCAGCTGAAAGAAGCC Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting PINK1 Purchased from Invitrogen Cat#1299001 siRNA targeting sequence: VCP #1: CAGUGUUGCUGAAAGGAAAGA UUUCCUUUCAGCAACACUGUG Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #2: GCAGCUAGCUCAGAUCAAAGA UUUGAUCUGAGCUAGCUGCUU Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #3: GCAAGAAGAUGGAUCUCAUUG AUGAGAUCCAUCUUCUUGCGG Synthesized by Tsingke Biotechnology (Beijing, China) N/A Recombinant DNA lentiCRISPR v2 Dr. Feng Zhang Addgene plasmid # 52961; RRID:Addgene_52961 Lentiguide V2-sgcGAS Dr. James Chen N/A Lentiguide V2-sgSTING Dr. James Chen N/A Lentiguide V2-sgTBK1 Dr. James Chen N/A pCDNA4-TREX1-WT-3XFLAG This paper N/A pCDNA4-PINK1-WT-3XFLAG This paper N/A pCDNA4-PINK1-E240K-3XFLAG This paper N/A pCDNA4-PINK1-G309D-3XFLAG This paper N/A Parkin-WT-FLAG Dr. Liming Wang N/A Parkin-S65A-FLAG Dr. Liming Wang N/A OPTN-FLAG Dr. Qiming Sun N/A TBK1-FLAG Dr. Wei Wan N/A Software and algorithms GraphPad Prism (https://www.graphpad. com/features) GraphPad Software Version 8.0 FlowJo (https://www.flowjo.com/) Tree Star Software Version 10.0 Adobe Illustrator (http://www.adobe.com/ cn/) Adobe Systems Version 26.0 ImageJ (https://imagej.nih.gov/ij/) Java Version 1.8.0 Other 1 × PBS BioChannel Biological Technology Co., Ltd. Cat#BC-BPBS-01 not-fat milk Biosharp Cat#BS102-500g Protease Inhibitor Cocktail TargetMol Cat#C0001-1 mL Phosphatase Inhibitor Cocktail III TargetMol Cat#C0004-2 mL Stripping Buffer CWBIO Cat#CW0056 Cell Reports 45, 117515, June 23, 2026 19
Article Title: A screen for cell envelope stress uncovers an inhibitor of prolipoprotein diacylglyceryl transferase, Lgt, in Escherichia coli .
Article Snippet: .. Bacterial and virus strains Refer to Data S7 This Work N/A Chemicals, peptides, and recombinant proteins Anhydrotetracycline Sigma-Aldrich Cat#37919 Spectinomycin Sigma-Aldrich Cat#S4014 Kanamycin Sigma-Aldrich Cat#60615 LOLCDE-in-1 MedChem Express CatHY-130839 Chloramphenicol Sigma-Aldrich Cat#C0378 CHIR-090 Sigma-Aldrich Cat#SML3092 SPR741 Spero Therapeutics and Northern Antibiotics N/A Diaminopimelic acid (DAP) Fisher Scientific Cat#AAB2239103 MOPS-minimal media TekNova Cat#M2101 .. from Sigma-Aldrich unless otherwise stated Sigma-Aldrich Variable Hexafluoroisopropylsulfamate (HFIPS) Enamine Cas# [637772-38-4] Dess-Martin periodinane AK Scientific Cas# [87413-09-0] 3-quinolinecarboxylic acid AK Scientific Cas# [6480-68-8] 2-picolinic acid AK Scientific Cas# [98-98-6] 4-(methylamino)benzoic acid AK Scientific Cas# [10541-83-0] 3,5-dimethoxybenzoic acid AK Scientific Cas# [1132-21-4] 2-thiophenecarboxaldehyde AK Scientific Cas# [98-03-3] 2-fluoro-4-methoxybenzoic acid AK Scientific Cas# [394-42-3] 3-amino-4-methoxybenzoic acid AK Scientific Cas# [2840-26-8] 4-(trifluoromethyl)benzoic acid AK Scientific Cas# [455-24-3] EDC$HCl AK Scientific Cas# [25952-53-8] DMAP AK Scientific Cas# [1122-58-3] Acetylthiophene Oakwood Chemical Cas# [88-15-3] hexafluoroisopropanol Oakwood Chemical Cas# [920-66-1] 2-Propionylthiophene AmBeed Cas# [13679-75-9] 4-chlorobenzoic acid AmBeed Cas# [74-11-3] 1-methyl-1H-imidazole-5-carboxylic acid AmBeed Cas# [41806-40-0] 2-pivaloylthiophene AmBeed Cas# [20409-48-7] 4-methoxybenzoic acid AmBeed Cas# [100-09-4]
Software:Article Title: A mobile CRISPRi collection enables genetic interaction studies for the essential genes of Escherichia coli
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli MFDpir Ferrières et al.50 N/A E. coli Keio gene deletion collection Baba et al.4 N/A E. coli BW25113 Baba et al.4 N/A Chemicals, peptides, and recombinant proteins Anhydrotetracycline Sigma-Aldrich Cat#37919 Spectinomycin Sigma-Aldrich Cat#S4014 Kanamycin Sigma-Aldrich Cat#60615 LOLCDE-in-1 MedChem Express CatHY-130839 MRL-494 GLPBio Cat#GC60254 Ampicillin Sigma-Aldrich Cat#A0166 Colistin Sigma-Aldrich Cat#C4461 Fosfomycin Sigma-Aldrich Cat#34089 Diaminopimelic acid (DAP) Fisher Scientific Cat#AAB2239103 MOPS-minimal media TekNova Cat#M2101 LB Broth, Miller Fisher Scientific Cat#DF0446 Agar Fisher Scientific Cat#BP1423 Deposited data Plate images and data from Dlpp CRISPRi Cross and pFD152-lolA Keio Cross This work https://doi.org/10.5281/zenodo.10214517 Oligonucleotides Oligonucleotides used in this work This paper See Table S1 Recombinant DNA pFD152 Depardieu and Bikard.44 Addgene Cat#125546 Software and algorithms GraphPad PRISM 9 GraphPad https://www.graphpad.com/ CRISPRbact sgRNA design tool Cui et al.34 https://gitlab.pasteur.fr/dbikard/crisprbact ImageJ ImageJ https://ImageJ.net/ij/ij/ ImageJ colony biomass quantification script French et al.12 https://doi.org/10.5281/zenodo.10214517 Analysis R code for Dlpp CRISPRi cross and CRISPRi Keio Crosses This work https://doi.org/10.5281/zenodo.10214517 Other Epson Perfection V750 high-resolution scanner Epson NA Singer ROTOR+ Singer Instruments https://www.singerinstruments.com/solution/rotor/ Singer RePads (384 Long and 1536 short) Singer Instruments Cat#REP-003; Cat#REP-005 Singer PlusPlates Singer Instruments Cat#PLU-003 Biotek Synergy Neo2 Plate Reader BioTek N/A ..
Activity Assay:
Injection:
Article Title: The NLRP3 inhibitor, MCC950, attenuates environmental pollutant PM2.5-induced acute lung injury by inhibiting alveolar macrophage pyroptosis
Article Snippet: Twelve hours prior to PM2.5 (8 mg/kg) administration, animals in the experimental groups were injected with MCC950 intraperitoneally [32] (50 mg/kg; MedChem Express Cat. No. HY‐12815, China) or dexamethasone [33] (1 mg/kg; Shiyaoyinhu, China). .. Twelve hours prior to PM2.5 (8 mg/kg) administration, animals in the experimental groups were injected with MCC950 intraperitoneally [32] (50 mg/kg; MedChem Express Cat. No. HY‐12815, China) or dexamethasone [33] (1 mg/kg; Shiyaoyinhu, China). ..
Bicinchoninic Acid Protein Assay:Article Title: STING-OPTN signaling confers cytoprotection through TBK1-dependent mitophagy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CCCP Sigma-Aldrich Cat#C2759 CCCP AmBeed Cat#A806566 Oligomycin Sigma-Aldrich Cat#11342 Antimycin A Sigma-Aldrich Cat#A8674 MG-132 Merck Millipore Cat#474790 Bafilomycin A1 Sigma-Aldrich Cat#B1793 Chloroquine Sigma-Aldrich Cat#C6628 NMS873 Sigma-Aldrich Cat#SML1128-5MG CB5083 MedChem Express CatHY-12861 CsA MedChem Express Cat#HY-B0579 VBIT4 MedChem Express Cat#HY-129122 BAI1 AmBeed Cat#A247314 RU.521 MedChem Express Cat#HY-114180 G150 AmBeed Cat#A1264491 C-170 MedChem Express Cat#HY-138682 C-171 MedChem Express Cat#HY-138683 GSK8612 MedChem Express Cat#HY-111941 Ferrostatin-1 MedChem Express Cat#HY-100579 Necrostatin-1 MedChem Express Cat#HY-15760 Z-VAD-FMK MedChem Express Cat#HY-16658B NAC Sigma-Aldrich Cat#A9165 LysoTrackerTM Deep Red ThermoFisher Cat#L12492 MitoSOX Red MedChem Express Cat#HY-D1055 Critical commercial assays BCA Protein Assay Kit New Cell & MolecularBiotech Cat#WB6501 ECL Chemiluminescent Substrate Reagent Kit Biosharp Cat#BL523B EndoFree Plasmid Midi Kit CWBIO Cat#CW2105S Gel Extraction Kit CWBIO Cat#CW2302M DNA Clean-up Kit CWBIO Cat#CW2301M NanoTransTM Transfection Reagent Plus CYTOCH Cat#CT0005 Lipofectamine 2000 Invitrogen Cat#52887 Lipofectamine 3000 Invitrogen Cat#100022052 Lipofectamine RNAiMAX Invitrogen Cat#56532 ClonExpress® II One Step Cloning Kit Vazyme Biotech Co., Ltd. Cat#C112 Cell Mitochondria Isolation Kit Beyotime Cat#C3601 Experimental models: Cell lines HeLa ATCC Cat#CCL-2; RRID:CVCL_0030 HeLa stably expressing YFP-Parkin (YPH) Dr. Richard J. Youle N/A YPH-cGAS KO This lab N/A YPH-STING KO This lab N/A YPH-TBK1 KO This lab N/A YPH-PINK1 KO Dr. Liming Wang N/A HeLa 3 plus Dr. Han-Ming Shen N/A HeLa 3 plus-cGAS KO This lab N/A HeLa 3 plus-STING KO This lab N/A HeLa 3 plus-TBK1 KO This lab N/A (Continued on next page) 18 Cell Reports 45, 117515, June 23, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER YPH mito-keima Dr. Richard J. Youle N/A HEK293T stably expressing YFP-Parkin Dr. Han-Ming Shen N/A SH-SY5Y ATCC Cat#CRL-2266; RRID:CVCL_0019 Oligonucleotides siRNA targeting cGAS Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.MB21D1.13 siRNA targeting TBK1 Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.TBK1.13 siRNA targeting STING #1: AGCATTACAACAACCTGCT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting STING #2: CGAACTTACAATCAGCATT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting OPTN: CCACCAGCTGAAAGAAGCC Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting PINK1 Purchased from Invitrogen Cat#1299001 siRNA targeting sequence: VCP #1: CAGUGUUGCUGAAAGGAAAGA UUUCCUUUCAGCAACACUGUG Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #2: GCAGCUAGCUCAGAUCAAAGA UUUGAUCUGAGCUAGCUGCUU Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #3: GCAAGAAGAUGGAUCUCAUUG AUGAGAUCCAUCUUCUUGCGG Synthesized by Tsingke Biotechnology (Beijing, China) N/A Recombinant DNA lentiCRISPR v2 Dr. Feng Zhang Addgene plasmid # 52961; RRID:Addgene_52961 Lentiguide V2-sgcGAS Dr. James Chen N/A Lentiguide V2-sgSTING Dr. James Chen N/A Lentiguide V2-sgTBK1 Dr. James Chen N/A pCDNA4-TREX1-WT-3XFLAG This paper N/A pCDNA4-PINK1-WT-3XFLAG This paper N/A pCDNA4-PINK1-E240K-3XFLAG This paper N/A pCDNA4-PINK1-G309D-3XFLAG This paper N/A Parkin-WT-FLAG Dr. Liming Wang N/A Parkin-S65A-FLAG Dr. Liming Wang N/A OPTN-FLAG Dr. Qiming Sun N/A TBK1-FLAG Dr. Wei Wan N/A Software and algorithms GraphPad Prism (https://www.graphpad. com/features) GraphPad Software Version 8.0 FlowJo (https://www.flowjo.com/) Tree Star Software Version 10.0 Adobe Illustrator (http://www.adobe.com/ cn/) Adobe Systems Version 26.0 ImageJ (https://imagej.nih.gov/ij/) Java Version 1.8.0 Other 1 × PBS BioChannel Biological Technology Co., Ltd. Cat#BC-BPBS-01 not-fat milk Biosharp Cat#BS102-500g Protease Inhibitor Cocktail TargetMol Cat#C0001-1 mL Phosphatase Inhibitor Cocktail III TargetMol Cat#C0004-2 mL Stripping Buffer CWBIO Cat#CW0056 Cell Reports 45, 117515, June 23, 2026 19
Plasmid Preparation:Article Title: STING-OPTN signaling confers cytoprotection through TBK1-dependent mitophagy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CCCP Sigma-Aldrich Cat#C2759 CCCP AmBeed Cat#A806566 Oligomycin Sigma-Aldrich Cat#11342 Antimycin A Sigma-Aldrich Cat#A8674 MG-132 Merck Millipore Cat#474790 Bafilomycin A1 Sigma-Aldrich Cat#B1793 Chloroquine Sigma-Aldrich Cat#C6628 NMS873 Sigma-Aldrich Cat#SML1128-5MG CB5083 MedChem Express CatHY-12861 CsA MedChem Express Cat#HY-B0579 VBIT4 MedChem Express Cat#HY-129122 BAI1 AmBeed Cat#A247314 RU.521 MedChem Express Cat#HY-114180 G150 AmBeed Cat#A1264491 C-170 MedChem Express Cat#HY-138682 C-171 MedChem Express Cat#HY-138683 GSK8612 MedChem Express Cat#HY-111941 Ferrostatin-1 MedChem Express Cat#HY-100579 Necrostatin-1 MedChem Express Cat#HY-15760 Z-VAD-FMK MedChem Express Cat#HY-16658B NAC Sigma-Aldrich Cat#A9165 LysoTrackerTM Deep Red ThermoFisher Cat#L12492 MitoSOX Red MedChem Express Cat#HY-D1055 Critical commercial assays BCA Protein Assay Kit New Cell & MolecularBiotech Cat#WB6501 ECL Chemiluminescent Substrate Reagent Kit Biosharp Cat#BL523B EndoFree Plasmid Midi Kit CWBIO Cat#CW2105S Gel Extraction Kit CWBIO Cat#CW2302M DNA Clean-up Kit CWBIO Cat#CW2301M NanoTransTM Transfection Reagent Plus CYTOCH Cat#CT0005 Lipofectamine 2000 Invitrogen Cat#52887 Lipofectamine 3000 Invitrogen Cat#100022052 Lipofectamine RNAiMAX Invitrogen Cat#56532 ClonExpress® II One Step Cloning Kit Vazyme Biotech Co., Ltd. Cat#C112 Cell Mitochondria Isolation Kit Beyotime Cat#C3601 Experimental models: Cell lines HeLa ATCC Cat#CCL-2; RRID:CVCL_0030 HeLa stably expressing YFP-Parkin (YPH) Dr. Richard J. Youle N/A YPH-cGAS KO This lab N/A YPH-STING KO This lab N/A YPH-TBK1 KO This lab N/A YPH-PINK1 KO Dr. Liming Wang N/A HeLa 3 plus Dr. Han-Ming Shen N/A HeLa 3 plus-cGAS KO This lab N/A HeLa 3 plus-STING KO This lab N/A HeLa 3 plus-TBK1 KO This lab N/A (Continued on next page) 18 Cell Reports 45, 117515, June 23, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER YPH mito-keima Dr. Richard J. Youle N/A HEK293T stably expressing YFP-Parkin Dr. Han-Ming Shen N/A SH-SY5Y ATCC Cat#CRL-2266; RRID:CVCL_0019 Oligonucleotides siRNA targeting cGAS Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.MB21D1.13 siRNA targeting TBK1 Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.TBK1.13 siRNA targeting STING #1: AGCATTACAACAACCTGCT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting STING #2: CGAACTTACAATCAGCATT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting OPTN: CCACCAGCTGAAAGAAGCC Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting PINK1 Purchased from Invitrogen Cat#1299001 siRNA targeting sequence: VCP #1: CAGUGUUGCUGAAAGGAAAGA UUUCCUUUCAGCAACACUGUG Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #2: GCAGCUAGCUCAGAUCAAAGA UUUGAUCUGAGCUAGCUGCUU Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #3: GCAAGAAGAUGGAUCUCAUUG AUGAGAUCCAUCUUCUUGCGG Synthesized by Tsingke Biotechnology (Beijing, China) N/A Recombinant DNA lentiCRISPR v2 Dr. Feng Zhang Addgene plasmid # 52961; RRID:Addgene_52961 Lentiguide V2-sgcGAS Dr. James Chen N/A Lentiguide V2-sgSTING Dr. James Chen N/A Lentiguide V2-sgTBK1 Dr. James Chen N/A pCDNA4-TREX1-WT-3XFLAG This paper N/A pCDNA4-PINK1-WT-3XFLAG This paper N/A pCDNA4-PINK1-E240K-3XFLAG This paper N/A pCDNA4-PINK1-G309D-3XFLAG This paper N/A Parkin-WT-FLAG Dr. Liming Wang N/A Parkin-S65A-FLAG Dr. Liming Wang N/A OPTN-FLAG Dr. Qiming Sun N/A TBK1-FLAG Dr. Wei Wan N/A Software and algorithms GraphPad Prism (https://www.graphpad. com/features) GraphPad Software Version 8.0 FlowJo (https://www.flowjo.com/) Tree Star Software Version 10.0 Adobe Illustrator (http://www.adobe.com/ cn/) Adobe Systems Version 26.0 ImageJ (https://imagej.nih.gov/ij/) Java Version 1.8.0 Other 1 × PBS BioChannel Biological Technology Co., Ltd. Cat#BC-BPBS-01 not-fat milk Biosharp Cat#BS102-500g Protease Inhibitor Cocktail TargetMol Cat#C0001-1 mL Phosphatase Inhibitor Cocktail III TargetMol Cat#C0004-2 mL Stripping Buffer CWBIO Cat#CW0056 Cell Reports 45, 117515, June 23, 2026 19
Gel Extraction:Article Title: STING-OPTN signaling confers cytoprotection through TBK1-dependent mitophagy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CCCP Sigma-Aldrich Cat#C2759 CCCP AmBeed Cat#A806566 Oligomycin Sigma-Aldrich Cat#11342 Antimycin A Sigma-Aldrich Cat#A8674 MG-132 Merck Millipore Cat#474790 Bafilomycin A1 Sigma-Aldrich Cat#B1793 Chloroquine Sigma-Aldrich Cat#C6628 NMS873 Sigma-Aldrich Cat#SML1128-5MG CB5083 MedChem Express CatHY-12861 CsA MedChem Express Cat#HY-B0579 VBIT4 MedChem Express Cat#HY-129122 BAI1 AmBeed Cat#A247314 RU.521 MedChem Express Cat#HY-114180 G150 AmBeed Cat#A1264491 C-170 MedChem Express Cat#HY-138682 C-171 MedChem Express Cat#HY-138683 GSK8612 MedChem Express Cat#HY-111941 Ferrostatin-1 MedChem Express Cat#HY-100579 Necrostatin-1 MedChem Express Cat#HY-15760 Z-VAD-FMK MedChem Express Cat#HY-16658B NAC Sigma-Aldrich Cat#A9165 LysoTrackerTM Deep Red ThermoFisher Cat#L12492 MitoSOX Red MedChem Express Cat#HY-D1055 Critical commercial assays BCA Protein Assay Kit New Cell & MolecularBiotech Cat#WB6501 ECL Chemiluminescent Substrate Reagent Kit Biosharp Cat#BL523B EndoFree Plasmid Midi Kit CWBIO Cat#CW2105S Gel Extraction Kit CWBIO Cat#CW2302M DNA Clean-up Kit CWBIO Cat#CW2301M NanoTransTM Transfection Reagent Plus CYTOCH Cat#CT0005 Lipofectamine 2000 Invitrogen Cat#52887 Lipofectamine 3000 Invitrogen Cat#100022052 Lipofectamine RNAiMAX Invitrogen Cat#56532 ClonExpress® II One Step Cloning Kit Vazyme Biotech Co., Ltd. Cat#C112 Cell Mitochondria Isolation Kit Beyotime Cat#C3601 Experimental models: Cell lines HeLa ATCC Cat#CCL-2; RRID:CVCL_0030 HeLa stably expressing YFP-Parkin (YPH) Dr. Richard J. Youle N/A YPH-cGAS KO This lab N/A YPH-STING KO This lab N/A YPH-TBK1 KO This lab N/A YPH-PINK1 KO Dr. Liming Wang N/A HeLa 3 plus Dr. Han-Ming Shen N/A HeLa 3 plus-cGAS KO This lab N/A HeLa 3 plus-STING KO This lab N/A HeLa 3 plus-TBK1 KO This lab N/A (Continued on next page) 18 Cell Reports 45, 117515, June 23, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER YPH mito-keima Dr. Richard J. Youle N/A HEK293T stably expressing YFP-Parkin Dr. Han-Ming Shen N/A SH-SY5Y ATCC Cat#CRL-2266; RRID:CVCL_0019 Oligonucleotides siRNA targeting cGAS Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.MB21D1.13 siRNA targeting TBK1 Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.TBK1.13 siRNA targeting STING #1: AGCATTACAACAACCTGCT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting STING #2: CGAACTTACAATCAGCATT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting OPTN: CCACCAGCTGAAAGAAGCC Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting PINK1 Purchased from Invitrogen Cat#1299001 siRNA targeting sequence: VCP #1: CAGUGUUGCUGAAAGGAAAGA UUUCCUUUCAGCAACACUGUG Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #2: GCAGCUAGCUCAGAUCAAAGA UUUGAUCUGAGCUAGCUGCUU Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #3: GCAAGAAGAUGGAUCUCAUUG AUGAGAUCCAUCUUCUUGCGG Synthesized by Tsingke Biotechnology (Beijing, China) N/A Recombinant DNA lentiCRISPR v2 Dr. Feng Zhang Addgene plasmid # 52961; RRID:Addgene_52961 Lentiguide V2-sgcGAS Dr. James Chen N/A Lentiguide V2-sgSTING Dr. James Chen N/A Lentiguide V2-sgTBK1 Dr. James Chen N/A pCDNA4-TREX1-WT-3XFLAG This paper N/A pCDNA4-PINK1-WT-3XFLAG This paper N/A pCDNA4-PINK1-E240K-3XFLAG This paper N/A pCDNA4-PINK1-G309D-3XFLAG This paper N/A Parkin-WT-FLAG Dr. Liming Wang N/A Parkin-S65A-FLAG Dr. Liming Wang N/A OPTN-FLAG Dr. Qiming Sun N/A TBK1-FLAG Dr. Wei Wan N/A Software and algorithms GraphPad Prism (https://www.graphpad. com/features) GraphPad Software Version 8.0 FlowJo (https://www.flowjo.com/) Tree Star Software Version 10.0 Adobe Illustrator (http://www.adobe.com/ cn/) Adobe Systems Version 26.0 ImageJ (https://imagej.nih.gov/ij/) Java Version 1.8.0 Other 1 × PBS BioChannel Biological Technology Co., Ltd. Cat#BC-BPBS-01 not-fat milk Biosharp Cat#BS102-500g Protease Inhibitor Cocktail TargetMol Cat#C0001-1 mL Phosphatase Inhibitor Cocktail III TargetMol Cat#C0004-2 mL Stripping Buffer CWBIO Cat#CW0056 Cell Reports 45, 117515, June 23, 2026 19
Transfection:Article Title: STING-OPTN signaling confers cytoprotection through TBK1-dependent mitophagy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CCCP Sigma-Aldrich Cat#C2759 CCCP AmBeed Cat#A806566 Oligomycin Sigma-Aldrich Cat#11342 Antimycin A Sigma-Aldrich Cat#A8674 MG-132 Merck Millipore Cat#474790 Bafilomycin A1 Sigma-Aldrich Cat#B1793 Chloroquine Sigma-Aldrich Cat#C6628 NMS873 Sigma-Aldrich Cat#SML1128-5MG CB5083 MedChem Express CatHY-12861 CsA MedChem Express Cat#HY-B0579 VBIT4 MedChem Express Cat#HY-129122 BAI1 AmBeed Cat#A247314 RU.521 MedChem Express Cat#HY-114180 G150 AmBeed Cat#A1264491 C-170 MedChem Express Cat#HY-138682 C-171 MedChem Express Cat#HY-138683 GSK8612 MedChem Express Cat#HY-111941 Ferrostatin-1 MedChem Express Cat#HY-100579 Necrostatin-1 MedChem Express Cat#HY-15760 Z-VAD-FMK MedChem Express Cat#HY-16658B NAC Sigma-Aldrich Cat#A9165 LysoTrackerTM Deep Red ThermoFisher Cat#L12492 MitoSOX Red MedChem Express Cat#HY-D1055 Critical commercial assays BCA Protein Assay Kit New Cell & MolecularBiotech Cat#WB6501 ECL Chemiluminescent Substrate Reagent Kit Biosharp Cat#BL523B EndoFree Plasmid Midi Kit CWBIO Cat#CW2105S Gel Extraction Kit CWBIO Cat#CW2302M DNA Clean-up Kit CWBIO Cat#CW2301M NanoTransTM Transfection Reagent Plus CYTOCH Cat#CT0005 Lipofectamine 2000 Invitrogen Cat#52887 Lipofectamine 3000 Invitrogen Cat#100022052 Lipofectamine RNAiMAX Invitrogen Cat#56532 ClonExpress® II One Step Cloning Kit Vazyme Biotech Co., Ltd. Cat#C112 Cell Mitochondria Isolation Kit Beyotime Cat#C3601 Experimental models: Cell lines HeLa ATCC Cat#CCL-2; RRID:CVCL_0030 HeLa stably expressing YFP-Parkin (YPH) Dr. Richard J. Youle N/A YPH-cGAS KO This lab N/A YPH-STING KO This lab N/A YPH-TBK1 KO This lab N/A YPH-PINK1 KO Dr. Liming Wang N/A HeLa 3 plus Dr. Han-Ming Shen N/A HeLa 3 plus-cGAS KO This lab N/A HeLa 3 plus-STING KO This lab N/A HeLa 3 plus-TBK1 KO This lab N/A (Continued on next page) 18 Cell Reports 45, 117515, June 23, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER YPH mito-keima Dr. Richard J. Youle N/A HEK293T stably expressing YFP-Parkin Dr. Han-Ming Shen N/A SH-SY5Y ATCC Cat#CRL-2266; RRID:CVCL_0019 Oligonucleotides siRNA targeting cGAS Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.MB21D1.13 siRNA targeting TBK1 Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.TBK1.13 siRNA targeting STING #1: AGCATTACAACAACCTGCT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting STING #2: CGAACTTACAATCAGCATT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting OPTN: CCACCAGCTGAAAGAAGCC Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting PINK1 Purchased from Invitrogen Cat#1299001 siRNA targeting sequence: VCP #1: CAGUGUUGCUGAAAGGAAAGA UUUCCUUUCAGCAACACUGUG Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #2: GCAGCUAGCUCAGAUCAAAGA UUUGAUCUGAGCUAGCUGCUU Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #3: GCAAGAAGAUGGAUCUCAUUG AUGAGAUCCAUCUUCUUGCGG Synthesized by Tsingke Biotechnology (Beijing, China) N/A Recombinant DNA lentiCRISPR v2 Dr. Feng Zhang Addgene plasmid # 52961; RRID:Addgene_52961 Lentiguide V2-sgcGAS Dr. James Chen N/A Lentiguide V2-sgSTING Dr. James Chen N/A Lentiguide V2-sgTBK1 Dr. James Chen N/A pCDNA4-TREX1-WT-3XFLAG This paper N/A pCDNA4-PINK1-WT-3XFLAG This paper N/A pCDNA4-PINK1-E240K-3XFLAG This paper N/A pCDNA4-PINK1-G309D-3XFLAG This paper N/A Parkin-WT-FLAG Dr. Liming Wang N/A Parkin-S65A-FLAG Dr. Liming Wang N/A OPTN-FLAG Dr. Qiming Sun N/A TBK1-FLAG Dr. Wei Wan N/A Software and algorithms GraphPad Prism (https://www.graphpad. com/features) GraphPad Software Version 8.0 FlowJo (https://www.flowjo.com/) Tree Star Software Version 10.0 Adobe Illustrator (http://www.adobe.com/ cn/) Adobe Systems Version 26.0 ImageJ (https://imagej.nih.gov/ij/) Java Version 1.8.0 Other 1 × PBS BioChannel Biological Technology Co., Ltd. Cat#BC-BPBS-01 not-fat milk Biosharp Cat#BS102-500g Protease Inhibitor Cocktail TargetMol Cat#C0001-1 mL Phosphatase Inhibitor Cocktail III TargetMol Cat#C0004-2 mL Stripping Buffer CWBIO Cat#CW0056 Cell Reports 45, 117515, June 23, 2026 19
Cloning:Article Title: STING-OPTN signaling confers cytoprotection through TBK1-dependent mitophagy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CCCP Sigma-Aldrich Cat#C2759 CCCP AmBeed Cat#A806566 Oligomycin Sigma-Aldrich Cat#11342 Antimycin A Sigma-Aldrich Cat#A8674 MG-132 Merck Millipore Cat#474790 Bafilomycin A1 Sigma-Aldrich Cat#B1793 Chloroquine Sigma-Aldrich Cat#C6628 NMS873 Sigma-Aldrich Cat#SML1128-5MG CB5083 MedChem Express CatHY-12861 CsA MedChem Express Cat#HY-B0579 VBIT4 MedChem Express Cat#HY-129122 BAI1 AmBeed Cat#A247314 RU.521 MedChem Express Cat#HY-114180 G150 AmBeed Cat#A1264491 C-170 MedChem Express Cat#HY-138682 C-171 MedChem Express Cat#HY-138683 GSK8612 MedChem Express Cat#HY-111941 Ferrostatin-1 MedChem Express Cat#HY-100579 Necrostatin-1 MedChem Express Cat#HY-15760 Z-VAD-FMK MedChem Express Cat#HY-16658B NAC Sigma-Aldrich Cat#A9165 LysoTrackerTM Deep Red ThermoFisher Cat#L12492 MitoSOX Red MedChem Express Cat#HY-D1055 Critical commercial assays BCA Protein Assay Kit New Cell & MolecularBiotech Cat#WB6501 ECL Chemiluminescent Substrate Reagent Kit Biosharp Cat#BL523B EndoFree Plasmid Midi Kit CWBIO Cat#CW2105S Gel Extraction Kit CWBIO Cat#CW2302M DNA Clean-up Kit CWBIO Cat#CW2301M NanoTransTM Transfection Reagent Plus CYTOCH Cat#CT0005 Lipofectamine 2000 Invitrogen Cat#52887 Lipofectamine 3000 Invitrogen Cat#100022052 Lipofectamine RNAiMAX Invitrogen Cat#56532 ClonExpress® II One Step Cloning Kit Vazyme Biotech Co., Ltd. Cat#C112 Cell Mitochondria Isolation Kit Beyotime Cat#C3601 Experimental models: Cell lines HeLa ATCC Cat#CCL-2; RRID:CVCL_0030 HeLa stably expressing YFP-Parkin (YPH) Dr. Richard J. Youle N/A YPH-cGAS KO This lab N/A YPH-STING KO This lab N/A YPH-TBK1 KO This lab N/A YPH-PINK1 KO Dr. Liming Wang N/A HeLa 3 plus Dr. Han-Ming Shen N/A HeLa 3 plus-cGAS KO This lab N/A HeLa 3 plus-STING KO This lab N/A HeLa 3 plus-TBK1 KO This lab N/A (Continued on next page) 18 Cell Reports 45, 117515, June 23, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER YPH mito-keima Dr. Richard J. Youle N/A HEK293T stably expressing YFP-Parkin Dr. Han-Ming Shen N/A SH-SY5Y ATCC Cat#CRL-2266; RRID:CVCL_0019 Oligonucleotides siRNA targeting cGAS Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.MB21D1.13 siRNA targeting TBK1 Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.TBK1.13 siRNA targeting STING #1: AGCATTACAACAACCTGCT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting STING #2: CGAACTTACAATCAGCATT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting OPTN: CCACCAGCTGAAAGAAGCC Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting PINK1 Purchased from Invitrogen Cat#1299001 siRNA targeting sequence: VCP #1: CAGUGUUGCUGAAAGGAAAGA UUUCCUUUCAGCAACACUGUG Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #2: GCAGCUAGCUCAGAUCAAAGA UUUGAUCUGAGCUAGCUGCUU Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #3: GCAAGAAGAUGGAUCUCAUUG AUGAGAUCCAUCUUCUUGCGG Synthesized by Tsingke Biotechnology (Beijing, China) N/A Recombinant DNA lentiCRISPR v2 Dr. Feng Zhang Addgene plasmid # 52961; RRID:Addgene_52961 Lentiguide V2-sgcGAS Dr. James Chen N/A Lentiguide V2-sgSTING Dr. James Chen N/A Lentiguide V2-sgTBK1 Dr. James Chen N/A pCDNA4-TREX1-WT-3XFLAG This paper N/A pCDNA4-PINK1-WT-3XFLAG This paper N/A pCDNA4-PINK1-E240K-3XFLAG This paper N/A pCDNA4-PINK1-G309D-3XFLAG This paper N/A Parkin-WT-FLAG Dr. Liming Wang N/A Parkin-S65A-FLAG Dr. Liming Wang N/A OPTN-FLAG Dr. Qiming Sun N/A TBK1-FLAG Dr. Wei Wan N/A Software and algorithms GraphPad Prism (https://www.graphpad. com/features) GraphPad Software Version 8.0 FlowJo (https://www.flowjo.com/) Tree Star Software Version 10.0 Adobe Illustrator (http://www.adobe.com/ cn/) Adobe Systems Version 26.0 ImageJ (https://imagej.nih.gov/ij/) Java Version 1.8.0 Other 1 × PBS BioChannel Biological Technology Co., Ltd. Cat#BC-BPBS-01 not-fat milk Biosharp Cat#BS102-500g Protease Inhibitor Cocktail TargetMol Cat#C0001-1 mL Phosphatase Inhibitor Cocktail III TargetMol Cat#C0004-2 mL Stripping Buffer CWBIO Cat#CW0056 Cell Reports 45, 117515, June 23, 2026 19
Isolation:Article Title: STING-OPTN signaling confers cytoprotection through TBK1-dependent mitophagy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CCCP Sigma-Aldrich Cat#C2759 CCCP AmBeed Cat#A806566 Oligomycin Sigma-Aldrich Cat#11342 Antimycin A Sigma-Aldrich Cat#A8674 MG-132 Merck Millipore Cat#474790 Bafilomycin A1 Sigma-Aldrich Cat#B1793 Chloroquine Sigma-Aldrich Cat#C6628 NMS873 Sigma-Aldrich Cat#SML1128-5MG CB5083 MedChem Express CatHY-12861 CsA MedChem Express Cat#HY-B0579 VBIT4 MedChem Express Cat#HY-129122 BAI1 AmBeed Cat#A247314 RU.521 MedChem Express Cat#HY-114180 G150 AmBeed Cat#A1264491 C-170 MedChem Express Cat#HY-138682 C-171 MedChem Express Cat#HY-138683 GSK8612 MedChem Express Cat#HY-111941 Ferrostatin-1 MedChem Express Cat#HY-100579 Necrostatin-1 MedChem Express Cat#HY-15760 Z-VAD-FMK MedChem Express Cat#HY-16658B NAC Sigma-Aldrich Cat#A9165 LysoTrackerTM Deep Red ThermoFisher Cat#L12492 MitoSOX Red MedChem Express Cat#HY-D1055 Critical commercial assays BCA Protein Assay Kit New Cell & MolecularBiotech Cat#WB6501 ECL Chemiluminescent Substrate Reagent Kit Biosharp Cat#BL523B EndoFree Plasmid Midi Kit CWBIO Cat#CW2105S Gel Extraction Kit CWBIO Cat#CW2302M DNA Clean-up Kit CWBIO Cat#CW2301M NanoTransTM Transfection Reagent Plus CYTOCH Cat#CT0005 Lipofectamine 2000 Invitrogen Cat#52887 Lipofectamine 3000 Invitrogen Cat#100022052 Lipofectamine RNAiMAX Invitrogen Cat#56532 ClonExpress® II One Step Cloning Kit Vazyme Biotech Co., Ltd. Cat#C112 Cell Mitochondria Isolation Kit Beyotime Cat#C3601 Experimental models: Cell lines HeLa ATCC Cat#CCL-2; RRID:CVCL_0030 HeLa stably expressing YFP-Parkin (YPH) Dr. Richard J. Youle N/A YPH-cGAS KO This lab N/A YPH-STING KO This lab N/A YPH-TBK1 KO This lab N/A YPH-PINK1 KO Dr. Liming Wang N/A HeLa 3 plus Dr. Han-Ming Shen N/A HeLa 3 plus-cGAS KO This lab N/A HeLa 3 plus-STING KO This lab N/A HeLa 3 plus-TBK1 KO This lab N/A (Continued on next page) 18 Cell Reports 45, 117515, June 23, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER YPH mito-keima Dr. Richard J. Youle N/A HEK293T stably expressing YFP-Parkin Dr. Han-Ming Shen N/A SH-SY5Y ATCC Cat#CRL-2266; RRID:CVCL_0019 Oligonucleotides siRNA targeting cGAS Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.MB21D1.13 siRNA targeting TBK1 Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.TBK1.13 siRNA targeting STING #1: AGCATTACAACAACCTGCT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting STING #2: CGAACTTACAATCAGCATT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting OPTN: CCACCAGCTGAAAGAAGCC Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting PINK1 Purchased from Invitrogen Cat#1299001 siRNA targeting sequence: VCP #1: CAGUGUUGCUGAAAGGAAAGA UUUCCUUUCAGCAACACUGUG Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #2: GCAGCUAGCUCAGAUCAAAGA UUUGAUCUGAGCUAGCUGCUU Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #3: GCAAGAAGAUGGAUCUCAUUG AUGAGAUCCAUCUUCUUGCGG Synthesized by Tsingke Biotechnology (Beijing, China) N/A Recombinant DNA lentiCRISPR v2 Dr. Feng Zhang Addgene plasmid # 52961; RRID:Addgene_52961 Lentiguide V2-sgcGAS Dr. James Chen N/A Lentiguide V2-sgSTING Dr. James Chen N/A Lentiguide V2-sgTBK1 Dr. James Chen N/A pCDNA4-TREX1-WT-3XFLAG This paper N/A pCDNA4-PINK1-WT-3XFLAG This paper N/A pCDNA4-PINK1-E240K-3XFLAG This paper N/A pCDNA4-PINK1-G309D-3XFLAG This paper N/A Parkin-WT-FLAG Dr. Liming Wang N/A Parkin-S65A-FLAG Dr. Liming Wang N/A OPTN-FLAG Dr. Qiming Sun N/A TBK1-FLAG Dr. Wei Wan N/A Software and algorithms GraphPad Prism (https://www.graphpad. com/features) GraphPad Software Version 8.0 FlowJo (https://www.flowjo.com/) Tree Star Software Version 10.0 Adobe Illustrator (http://www.adobe.com/ cn/) Adobe Systems Version 26.0 ImageJ (https://imagej.nih.gov/ij/) Java Version 1.8.0 Other 1 × PBS BioChannel Biological Technology Co., Ltd. Cat#BC-BPBS-01 not-fat milk Biosharp Cat#BS102-500g Protease Inhibitor Cocktail TargetMol Cat#C0001-1 mL Phosphatase Inhibitor Cocktail III TargetMol Cat#C0004-2 mL Stripping Buffer CWBIO Cat#CW0056 Cell Reports 45, 117515, June 23, 2026 19
Stable Transfection:Article Title: STING-OPTN signaling confers cytoprotection through TBK1-dependent mitophagy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CCCP Sigma-Aldrich Cat#C2759 CCCP AmBeed Cat#A806566 Oligomycin Sigma-Aldrich Cat#11342 Antimycin A Sigma-Aldrich Cat#A8674 MG-132 Merck Millipore Cat#474790 Bafilomycin A1 Sigma-Aldrich Cat#B1793 Chloroquine Sigma-Aldrich Cat#C6628 NMS873 Sigma-Aldrich Cat#SML1128-5MG CB5083 MedChem Express CatHY-12861 CsA MedChem Express Cat#HY-B0579 VBIT4 MedChem Express Cat#HY-129122 BAI1 AmBeed Cat#A247314 RU.521 MedChem Express Cat#HY-114180 G150 AmBeed Cat#A1264491 C-170 MedChem Express Cat#HY-138682 C-171 MedChem Express Cat#HY-138683 GSK8612 MedChem Express Cat#HY-111941 Ferrostatin-1 MedChem Express Cat#HY-100579 Necrostatin-1 MedChem Express Cat#HY-15760 Z-VAD-FMK MedChem Express Cat#HY-16658B NAC Sigma-Aldrich Cat#A9165 LysoTrackerTM Deep Red ThermoFisher Cat#L12492 MitoSOX Red MedChem Express Cat#HY-D1055 Critical commercial assays BCA Protein Assay Kit New Cell & MolecularBiotech Cat#WB6501 ECL Chemiluminescent Substrate Reagent Kit Biosharp Cat#BL523B EndoFree Plasmid Midi Kit CWBIO Cat#CW2105S Gel Extraction Kit CWBIO Cat#CW2302M DNA Clean-up Kit CWBIO Cat#CW2301M NanoTransTM Transfection Reagent Plus CYTOCH Cat#CT0005 Lipofectamine 2000 Invitrogen Cat#52887 Lipofectamine 3000 Invitrogen Cat#100022052 Lipofectamine RNAiMAX Invitrogen Cat#56532 ClonExpress® II One Step Cloning Kit Vazyme Biotech Co., Ltd. Cat#C112 Cell Mitochondria Isolation Kit Beyotime Cat#C3601 Experimental models: Cell lines HeLa ATCC Cat#CCL-2; RRID:CVCL_0030 HeLa stably expressing YFP-Parkin (YPH) Dr. Richard J. Youle N/A YPH-cGAS KO This lab N/A YPH-STING KO This lab N/A YPH-TBK1 KO This lab N/A YPH-PINK1 KO Dr. Liming Wang N/A HeLa 3 plus Dr. Han-Ming Shen N/A HeLa 3 plus-cGAS KO This lab N/A HeLa 3 plus-STING KO This lab N/A HeLa 3 plus-TBK1 KO This lab N/A (Continued on next page) 18 Cell Reports 45, 117515, June 23, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER YPH mito-keima Dr. Richard J. Youle N/A HEK293T stably expressing YFP-Parkin Dr. Han-Ming Shen N/A SH-SY5Y ATCC Cat#CRL-2266; RRID:CVCL_0019 Oligonucleotides siRNA targeting cGAS Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.MB21D1.13 siRNA targeting TBK1 Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.TBK1.13 siRNA targeting STING #1: AGCATTACAACAACCTGCT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting STING #2: CGAACTTACAATCAGCATT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting OPTN: CCACCAGCTGAAAGAAGCC Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting PINK1 Purchased from Invitrogen Cat#1299001 siRNA targeting sequence: VCP #1: CAGUGUUGCUGAAAGGAAAGA UUUCCUUUCAGCAACACUGUG Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #2: GCAGCUAGCUCAGAUCAAAGA UUUGAUCUGAGCUAGCUGCUU Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #3: GCAAGAAGAUGGAUCUCAUUG AUGAGAUCCAUCUUCUUGCGG Synthesized by Tsingke Biotechnology (Beijing, China) N/A Recombinant DNA lentiCRISPR v2 Dr. Feng Zhang Addgene plasmid # 52961; RRID:Addgene_52961 Lentiguide V2-sgcGAS Dr. James Chen N/A Lentiguide V2-sgSTING Dr. James Chen N/A Lentiguide V2-sgTBK1 Dr. James Chen N/A pCDNA4-TREX1-WT-3XFLAG This paper N/A pCDNA4-PINK1-WT-3XFLAG This paper N/A pCDNA4-PINK1-E240K-3XFLAG This paper N/A pCDNA4-PINK1-G309D-3XFLAG This paper N/A Parkin-WT-FLAG Dr. Liming Wang N/A Parkin-S65A-FLAG Dr. Liming Wang N/A OPTN-FLAG Dr. Qiming Sun N/A TBK1-FLAG Dr. Wei Wan N/A Software and algorithms GraphPad Prism (https://www.graphpad. com/features) GraphPad Software Version 8.0 FlowJo (https://www.flowjo.com/) Tree Star Software Version 10.0 Adobe Illustrator (http://www.adobe.com/ cn/) Adobe Systems Version 26.0 ImageJ (https://imagej.nih.gov/ij/) Java Version 1.8.0 Other 1 × PBS BioChannel Biological Technology Co., Ltd. Cat#BC-BPBS-01 not-fat milk Biosharp Cat#BS102-500g Protease Inhibitor Cocktail TargetMol Cat#C0001-1 mL Phosphatase Inhibitor Cocktail III TargetMol Cat#C0004-2 mL Stripping Buffer CWBIO Cat#CW0056 Cell Reports 45, 117515, June 23, 2026 19
Expressing:Article Title: STING-OPTN signaling confers cytoprotection through TBK1-dependent mitophagy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins CCCP Sigma-Aldrich Cat#C2759 CCCP AmBeed Cat#A806566 Oligomycin Sigma-Aldrich Cat#11342 Antimycin A Sigma-Aldrich Cat#A8674 MG-132 Merck Millipore Cat#474790 Bafilomycin A1 Sigma-Aldrich Cat#B1793 Chloroquine Sigma-Aldrich Cat#C6628 NMS873 Sigma-Aldrich Cat#SML1128-5MG CB5083 MedChem Express CatHY-12861 CsA MedChem Express Cat#HY-B0579 VBIT4 MedChem Express Cat#HY-129122 BAI1 AmBeed Cat#A247314 RU.521 MedChem Express Cat#HY-114180 G150 AmBeed Cat#A1264491 C-170 MedChem Express Cat#HY-138682 C-171 MedChem Express Cat#HY-138683 GSK8612 MedChem Express Cat#HY-111941 Ferrostatin-1 MedChem Express Cat#HY-100579 Necrostatin-1 MedChem Express Cat#HY-15760 Z-VAD-FMK MedChem Express Cat#HY-16658B NAC Sigma-Aldrich Cat#A9165 LysoTrackerTM Deep Red ThermoFisher Cat#L12492 MitoSOX Red MedChem Express Cat#HY-D1055 Critical commercial assays BCA Protein Assay Kit New Cell & MolecularBiotech Cat#WB6501 ECL Chemiluminescent Substrate Reagent Kit Biosharp Cat#BL523B EndoFree Plasmid Midi Kit CWBIO Cat#CW2105S Gel Extraction Kit CWBIO Cat#CW2302M DNA Clean-up Kit CWBIO Cat#CW2301M NanoTransTM Transfection Reagent Plus CYTOCH Cat#CT0005 Lipofectamine 2000 Invitrogen Cat#52887 Lipofectamine 3000 Invitrogen Cat#100022052 Lipofectamine RNAiMAX Invitrogen Cat#56532 ClonExpress® II One Step Cloning Kit Vazyme Biotech Co., Ltd. Cat#C112 Cell Mitochondria Isolation Kit Beyotime Cat#C3601 Experimental models: Cell lines HeLa ATCC Cat#CCL-2; RRID:CVCL_0030 HeLa stably expressing YFP-Parkin (YPH) Dr. Richard J. Youle N/A YPH-cGAS KO This lab N/A YPH-STING KO This lab N/A YPH-TBK1 KO This lab N/A YPH-PINK1 KO Dr. Liming Wang N/A HeLa 3 plus Dr. Han-Ming Shen N/A HeLa 3 plus-cGAS KO This lab N/A HeLa 3 plus-STING KO This lab N/A HeLa 3 plus-TBK1 KO This lab N/A (Continued on next page) 18 Cell Reports 45, 117515, June 23, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER YPH mito-keima Dr. Richard J. Youle N/A HEK293T stably expressing YFP-Parkin Dr. Han-Ming Shen N/A SH-SY5Y ATCC Cat#CRL-2266; RRID:CVCL_0019 Oligonucleotides siRNA targeting cGAS Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.MB21D1.13 siRNA targeting TBK1 Purchased from Integrated DNA Technologies (IDT) Design ID: hs.Ri.TBK1.13 siRNA targeting STING #1: AGCATTACAACAACCTGCT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting STING #2: CGAACTTACAATCAGCATT Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting OPTN: CCACCAGCTGAAAGAAGCC Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting PINK1 Purchased from Invitrogen Cat#1299001 siRNA targeting sequence: VCP #1: CAGUGUUGCUGAAAGGAAAGA UUUCCUUUCAGCAACACUGUG Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #2: GCAGCUAGCUCAGAUCAAAGA UUUGAUCUGAGCUAGCUGCUU Synthesized by Tsingke Biotechnology (Beijing, China) N/A siRNA targeting sequence: VCP #3: GCAAGAAGAUGGAUCUCAUUG AUGAGAUCCAUCUUCUUGCGG Synthesized by Tsingke Biotechnology (Beijing, China) N/A Recombinant DNA lentiCRISPR v2 Dr. Feng Zhang Addgene plasmid # 52961; RRID:Addgene_52961 Lentiguide V2-sgcGAS Dr. James Chen N/A Lentiguide V2-sgSTING Dr. James Chen N/A Lentiguide V2-sgTBK1 Dr. James Chen N/A pCDNA4-TREX1-WT-3XFLAG This paper N/A pCDNA4-PINK1-WT-3XFLAG This paper N/A pCDNA4-PINK1-E240K-3XFLAG This paper N/A pCDNA4-PINK1-G309D-3XFLAG This paper N/A Parkin-WT-FLAG Dr. Liming Wang N/A Parkin-S65A-FLAG Dr. Liming Wang N/A OPTN-FLAG Dr. Qiming Sun N/A TBK1-FLAG Dr. Wei Wan N/A Software and algorithms GraphPad Prism (https://www.graphpad. com/features) GraphPad Software Version 8.0 FlowJo (https://www.flowjo.com/) Tree Star Software Version 10.0 Adobe Illustrator (http://www.adobe.com/ cn/) Adobe Systems Version 26.0 ImageJ (https://imagej.nih.gov/ij/) Java Version 1.8.0 Other 1 × PBS BioChannel Biological Technology Co., Ltd. Cat#BC-BPBS-01 not-fat milk Biosharp Cat#BS102-500g Protease Inhibitor Cocktail TargetMol Cat#C0001-1 mL Phosphatase Inhibitor Cocktail III TargetMol Cat#C0004-2 mL Stripping Buffer CWBIO Cat#CW0056 Cell Reports 45, 117515, June 23, 2026 19
Magnetic Beads:
Multiplex sample analysis:
BIA-KA:
cDNA Synthesis:
Mass Spectrometry:
Northern Blot:Article Title: A screen for cell envelope stress uncovers an inhibitor of prolipoprotein diacylglyceryl transferase, Lgt, in Escherichia coli .
Article Snippet: .. Bacterial and virus strains Refer to Data S7 This Work N/A Chemicals, peptides, and recombinant proteins Anhydrotetracycline Sigma-Aldrich Cat#37919 Spectinomycin Sigma-Aldrich Cat#S4014 Kanamycin Sigma-Aldrich Cat#60615 LOLCDE-in-1 MedChem Express CatHY-130839 Chloramphenicol Sigma-Aldrich Cat#C0378 CHIR-090 Sigma-Aldrich Cat#SML3092 SPR741 Spero Therapeutics and Northern Antibiotics N/A Diaminopimelic acid (DAP) Fisher Scientific Cat#AAB2239103 MOPS-minimal media TekNova Cat#M2101 .. from Sigma-Aldrich unless otherwise stated Sigma-Aldrich Variable Hexafluoroisopropylsulfamate (HFIPS) Enamine Cas# [637772-38-4] Dess-Martin periodinane AK Scientific Cas# [87413-09-0] 3-quinolinecarboxylic acid AK Scientific Cas# [6480-68-8] 2-picolinic acid AK Scientific Cas# [98-98-6] 4-(methylamino)benzoic acid AK Scientific Cas# [10541-83-0] 3,5-dimethoxybenzoic acid AK Scientific Cas# [1132-21-4] 2-thiophenecarboxaldehyde AK Scientific Cas# [98-03-3] 2-fluoro-4-methoxybenzoic acid AK Scientific Cas# [394-42-3] 3-amino-4-methoxybenzoic acid AK Scientific Cas# [2840-26-8] 4-(trifluoromethyl)benzoic acid AK Scientific Cas# [455-24-3] EDC$HCl AK Scientific Cas# [25952-53-8] DMAP AK Scientific Cas# [1122-58-3] Acetylthiophene Oakwood Chemical Cas# [88-15-3] hexafluoroisopropanol Oakwood Chemical Cas# [920-66-1] 2-Propionylthiophene AmBeed Cas# [13679-75-9] 4-chlorobenzoic acid AmBeed Cas# [74-11-3] 1-methyl-1H-imidazole-5-carboxylic acid AmBeed Cas# [41806-40-0] 2-pivaloylthiophene AmBeed Cas# [20409-48-7] 4-methoxybenzoic acid AmBeed Cas# [100-09-4]
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